Please note: This program is hosted by UC Santa Cruz but is not maintained by them. The author can be reached at jeltje.van.baren at gmail.com
The original Primer3 program designs
primersets for one sequence and it allows you to change almost every
parameter. The PCR Suite has more stringent settings (to allow more
working primersets) and a smaller range of parameters (because you won't
change most of them anyway).
The suite consists of four programs:
|Overlapping_Primers||is for creating multiple overlapping PCR products in one sequence.|
|Genomic_Primers||designs primers around exons in genomic sequence. All you need is a GenBank file containing your gene.|
|SNP_Primers||designs primers around every SNP in a GenBank file.|
|cDNA_Primers||designs primers around open reading frames. Simply upload a GenBank file containing your genes.|
If you want to know more about using the PCR Suite, if something doesn't work,
or if you have comments or suggestions, please
If these programs have been useful to you, please cite:
The PCR Suite M.J. van Baren and P. Heutink. Bioinformatics. 2004 Mar 1;20:591-3
This software is based on the Primer3 program
developed by the Whitehead institute. The Primer3 core program used by
PCR Suite was developed by the Whitehead Institute and used "as is".
See the copyright notice and
for PCR Suite and the Whitehead's Primer3
The source code for PCR Suite is available here.
A detailed description of how PCR Suite interacts with Primer3 can be found here.