cDNA Primers - Input form for Primer3

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This is an input form for creating primers around the Open Reading Frame of cDNAs. For primer design, the Primer3 program is used. You can change the default settings below. The input file is a list of GenBank sequences.

Select the GenBank file that contains your cDNAs How do I obtain a list of GenBank sequences?
Product Size Range Specify the minimum and maximum size of your product.



General Primer Picking Conditions

Primer Size Min: Opt: Max:
Primer Tm Min: Opt: Max: Max Tm Difference:
Primer GC% Min:   Max:
Max Self Complementarity: Max 3' Self Complementarity:
CG Clamp: Max Poly-X:

















This software is based on the Primer3 program developed by the Whitehead institute. The Primer3 core program used by PCR Suite was developed by the Whitehead Institute and used "as is".
See the copyright notice and disclaimer for PCR Suite and the Whitehead's Primer3