Overlapping Primersets - Input form for Primer3

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This is an input form for creating overlapping PCR products in large sequences. Just paste your sequence below and select the minimum and maximum overlap. For primer design, the Primer3 program is used. You can change the default settings below.

Target Which region of the sequence above needs to be covered?
use the format "start location", "length of target", eg 50,100
Product Size Range Specify the minimum and maximum size of your product.
Overlap Size Range What is the minimum and maximum size of the overlap between products?
(This is including the primers)

General Primer Picking Conditions - you don't need to change these, but you can

Primer Size Min: Opt: Max:
Primer Tm Min: Opt: Max: Max Tm Difference:
Primer GC% Min:   Max:
Max Self Complementarity: Max 3' Self Complementarity:
CG Clamp: Max Poly-X:

This software is based on the Primer3 program developed by the Whitehead institute. The Primer3 core program used by PCR Suite was developed by the Whitehead Institute and used "as is".
See the copyright notice and disclaimer for PCR Suite and the Whitehead's Primer3